MCU lost communication

Your error actually is:

b'Got EOF when reading from device'
Timeout with MCU 'mcu' (eventtime=27791.026172)

See Timeout with MCU / Lost communication with MCU
Unfortunately an ugly error to get rid of, but in all cases I’m aware and all cases that have been successfully solved here it was related to hardware instabilities.

Also remove other USB devices that you might have connected.

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